FastQCFastQC Report
Thu 28 Mar 2019
1530_Read1.fq

Summary

[OK] Basic Statistics

Measure Value
Filename 1530_Read1.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 55605619
Filtered Sequences 0
Sequence length 75
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG 3734780 6.7165514334081955 TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG 79548 0.1430574848919495 TruSeq Adapter, Index 5 (98% over 63bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACAC 18865800 5.7545877 100.56842 12
AAAAA 46593490 5.505951 40.781437 66
GAAAA 25453040 4.186632 55.975525 63
TTTTC 21811750 3.985155 6.004756 12
CAGTG 11635605 3.7653208 105.60204 35
TCCAG 11776345 3.75563 104.630554 25
GTGTG 10442240 3.5329707 8.74508 21
CTGAA 14604405 3.3953056 77.22915 19
ACACA 14849975 3.3020787 73.3625 32
CGTCT 9975880 3.2780538 106.013985 16
TTTCT 17686440 3.231433 6.234968 27
CTCCA 10074300 3.166259 102.40951 24
TGAAA 18458780 3.1283884 57.92399 62
GGAAG 9669245 3.08144 103.925575 5
CACAG 9955055 3.0812247 99.975006 33
GATCG 9044335 2.9267771 110.856544 1
GCACA 9370590 2.9003248 101.03306 11
CACGT 9072895 2.8934646 104.25522 14
TCTGC 8663225 2.8467183 106.568344 56
GATCT 11726180 2.8089507 73.28633 1
CTGCT 8509580 2.7962306 103.44561 57
TGTGT 11129590 2.787405 5.3451552 36
TTCTG 11278930 2.7838643 78.49009 55
GTGGA 8421290 2.765237 7.074621 39
CCTGG 6194945 2.7499688 7.283201 18
CTTCT 11212270 2.7273004 77.29322 54
AGAGC 8588470 2.6973436 102.64917 8
GAGCA 8542215 2.6828165 102.45539 9
GTCTG 8040045 2.680798 108.39329 17
CCAGT 8319455 2.6531827 102.97824 26
TCTTC 10786665 2.623775 77.30706 53
TTTCC 10737225 2.6117492 5.1211567 50
TCCAC 8284725 2.6038125 10.9562025 3
TTCTC 10672395 2.5959797 5.3555274 29
TTCCA 10941845 2.5830772 5.489504 51
AGCAC 8127655 2.5156198 100.861275 10
GAAGA 10899430 2.4954388 75.10941 6
CAGTC 7761095 2.4751146 102.91031 27
TCTGA 10265395 2.4590268 78.16794 18
CTTGA 10123095 2.4249396 76.13099 60
ACGTC 7591245 2.420947 103.87765 15
ACTCC 7698200 2.419473 101.73191 23
GAGAA 10494080 2.4026334 5.0060115 29
ATGCC 7404345 2.3613424 101.70562 47
AAGAG 10293400 2.3566873 75.20634 7
AAAGG 10286675 2.3551476 71.424644 71
GTGAT 9530925 2.3166656 77.02978 37
GCTTG 6874645 2.292218 105.15709 59
TCTCT 9349625 2.274226 7.7074714 3
AGTGA 9628385 2.2713766 75.620834 36
GTCCT 6903410 2.2684467 5.2084527 13
TGCCG 5052680 2.2429116 140.52505 48
ACAGT 9637060 2.2404723 75.0394 34
TCACA 9774630 2.2395182 74.45428 30
GCCAT 6962350 2.2203844 5.278625 1
AGGAC 7041965 2.2116394 6.806278 66
CGGAA 6990500 2.195476 101.84113 4
CTGGG 4871710 2.1943834 7.485921 19
CCGTC 5012935 2.1930153 138.00107 50
GCCGT 4907160 2.1783142 140.2156 49
GTCAC 6815890 2.1736765 102.74654 29
TCTCA 9178525 2.1668046 6.5519423 3
ATGGC 6576990 2.1283362 5.864689 12
GGTGG 4645620 2.12332 6.3111186 69
CCTCC 4888775 2.1077003 8.443049 4
CTCTG 6386550 2.0986073 5.345491 61
CTCTC 6454660 2.0902467 5.7774653 14
CCCTG 4737605 2.0725665 7.902842 4
AGTGG 6310190 2.0720305 6.20207 57
ATCTC 8754955 2.0668108 71.72055 40
TCCTC 6353270 2.0574129 10.698348 3
TCTCG 6255645 2.0555923 100.10265 41
GATCC 6442550 2.0546134 98.32074 1
CCCAG 4834115 2.0524566 6.3659253 70
TGCTT 8303115 2.049374 77.8696 58
GTCTT 8216985 2.0281155 78.10907 52
AACTC 8745010 2.0036163 74.50183 22
GAACT 8527385 1.9824893 75.61419 21
CAGAG 6242035 1.9604089 5.803199 54
AAAAG 11867135 1.9519604 51.98618 70
CCACC 4643940 1.9431347 7.2807655 4
CCACT 6142090 1.930402 5.442654 29
GAGGA 5974880 1.9041026 5.794148 32
CTGTG 5701865 1.9011769 5.778701 19
AGTCA 8173755 1.9002757 75.2041 28
CAGGG 4333735 1.8945272 5.781024 6
CTCCT 5771055 1.8688712 5.5625224 16
TCGGA 5741080 1.8578328 104.73056 3
ATCGG 5672185 1.8355383 105.74539 2
GCTGG 4034360 1.8172125 7.482436 27
ATCCA 7883395 1.8062072 28.81138 2
ACACG 5789540 1.7919413 100.127075 13
TGAAC 7693120 1.7885352 75.36853 20
TCCAT 7557680 1.7841663 11.6874075 3
TCCTT 7331695 1.7833797 8.731483 3
TTGAA 10060260 1.7567885 56.170708 61
CCTTC 5392905 1.7464129 5.045684 4
GGAGG 3909410 1.734165 6.089992 19
CCAGC 4082610 1.7333845 6.987879 4
CTGAG 5294765 1.7134037 5.683926 30
CCTCT 5266675 1.7055352 5.450675 13
GTATG 6979980 1.6966119 77.464226 45
TCCTG 5160485 1.6957248 10.821854 3
TGCTG 5049260 1.6835784 5.947525 26
CTGGC 3779145 1.6775824 5.539883 28
TCTGT 6772895 1.6716852 6.400078 3
CATCC 5302190 1.6664292 8.916079 5
GGCAG 3796260 1.6595656 6.285997 14
TCTGG 4971870 1.6577742 6.2943497 3
CCATA 7216985 1.6535224 5.1117105 9
CCAGA 5320640 1.6468104 5.2746506 53
TATGC 6871265 1.645979 76.262054 46
CTCGT 4959240 1.6295961 100.35142 42
TCCCA 5105560 1.6046301 8.294684 3
TCAAG 6880185 1.59954 8.915595 3
GTGGG 3496990 1.598329 5.8295913 70
GGACC 3662090 1.5777086 7.8281145 67
GCCAG 3613305 1.5566908 8.153536 40
TGTCC 4725870 1.5529113 5.510891 19
TCTTT 8482025 1.5497236 9.2732725 3
TGATC 6461825 1.5478995 72.72381 38
TCCCT 4739360 1.5347719 11.694258 3
TGGAG 4670585 1.5336457 5.0994434 40
TCTCC 4714175 1.5266162 7.1455708 3
AGGTG 4642060 1.5242792 5.092598 13
TCCCC 3518395 1.5168877 8.190424 3
GGTCC 3371165 1.496478 6.998162 28
GCTCC 3413165 1.4931618 6.327361 47
GCCTG 3351175 1.4876043 5.1029425 70
TCAGG 4595825 1.4872246 5.74194 3
AGCTG 4485105 1.451395 5.032914 13
GCCCC 2441540 1.4219952 6.779448 48
GATCA 6096535 1.4173532 59.62321 1
TCCTA 5974945 1.4105248 5.091121 3
TGGTG 4122155 1.3946675 5.818895 19
CCATC 4400740 1.3831118 9.695733 4
GTGAG 4172420 1.370067 5.0433526 21
GCAAG 4327095 1.358992 6.4300485 50
GGGCA 3103295 1.3566304 6.4926085 21
GGCCC 2288985 1.3527516 7.780249 47
GGAGC 3091345 1.3514063 5.645413 22
CACCA 4416245 1.347076 5.8346167 33
TGGGC 2970810 1.3381535 6.5175834 20
TGAGG 4017275 1.319123 5.307834 31
AGGCC 3044535 1.3116524 5.9224706 46
GCTGA 3930025 1.2717693 5.364758 28
GTCCA 3974720 1.26759 5.120996 20
CAAGG 4006855 1.2584155 5.024609 4
ATCAC 5490625 1.2579868 11.921855 2
ATCCC 3949890 1.241414 24.245258 2
ATCTG 5155570 1.2349923 19.014454 2
GTCTC 3742200 1.2296795 7.9020324 59
CGTAT 5087655 1.2187237 75.86788 44
ATCCT 5082285 1.1997917 24.767813 2
CCCAT 3775630 1.1866456 6.5679193 8
TCAGC 3688520 1.1763173 5.9616847 15
TCCAA 5131700 1.1757514 7.014587 21
TCGTA 4899250 1.1735921 75.35488 43
TCATG 4805240 1.1510724 5.4030538 3
AGGGC 2607670 1.139964 5.2408457 7
CTGAC 3505120 1.1178285 5.452969 29
ATCTT 6167500 1.0936322 20.993387 2
GTGGT 3217860 1.0887132 5.125064 58
ATCAG 4595125 1.0682977 12.305138 2
GACAC 3415540 1.0571561 6.2637396 31
ACACC 3223040 0.9831158 5.4703746 32
ATCAT 5238355 0.90149736 11.288941 2
TCAAA 5174690 0.8642936 6.2457776 3
GACCC 2002120 0.8500552 6.8262267 68
ATCAA 5034315 0.84084773 16.261328 2
CTAGC 2468895 0.78736293 5.2446017 47
TCTTA 3921285 0.6953293 5.23234 3
ATCTA 3618775 0.6227749 8.103573 2
CCCGG 1018495 0.6019134 5.2998695 50
CCCCG 990770 0.5770417 5.274226 49
CCGCC 803820 0.4681587 5.4604783 38
CCGGC 662265 0.39138743 5.1306205 51
Produced by FastQC (version 0.10.1)