Running the workflow

The workflow is in the center panel; for its input we select the same results from Part 1 that we used for the join with the PolyPhen-2 predictions (red arrow). Several of the steps have annotations explaining what the workflow is doing. The annotation for step 10 tells us that column 5 of the output is what we want to submit to SIFT, and that column 4 has the original allele data (blue arrow). There are 10 steps in the workflow, all of which will be run when you click the "Run workflow" button (green arrow).

[screen shot]